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qpcr primer design software getprime  (PrimerDesign Inc)

 
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    PrimerDesign Inc qpcr primer design software getprime
    JBrowse-based graphical view of <t>GETPrime</t> primer pairs targeting the Rbbp9 mouse gene. The blue boxes on the left are the available tracks that can be dragged in the JBrowse genome view . In this example, the transcripts, the gene-specific primers (covering the majority of splice variants if possible) and the transcript-specific primers (covering a single splice variant, when possible) have been dragged into the browser. The upper part of the figure shows tools to zoom, to move to up- or downstream of the genome location, and to enter another chromosome, another position on the chromosome or also an Ensembl ID. Each primer is annotated by its Ensembl ID, its iteration in GETPrime (e.g. −1), its ranking (e.g. _3) and its primer type (forward and reverse primers are abbreviated Fwd and Rv, respectively). The blue box for each primer represents the respective alignment to the transcripts and sometimes a thin line between two blue boxes is used to bridge an intron region for primers spanning two exons. The primer pairs in the gene-specific track cover both transcripts. The primer pairs from the first iteration (‘−1’) and the second iteration (‘−2’) in the transcript-specific track are specific to the largest transcript Rbbp9-001 and the shortest transcript Rbbp9-002 , respectively.
    Qpcr Primer Design Software Getprime, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qpcr+primer+design+software+getprime/pmc03173022-43-13-10?v=PrimerDesign+Inc
    Average 90 stars, based on 1 article reviews
    qpcr primer design software getprime - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "GETPrime: a gene- or transcript-specific primer database for quantitative real-time PCR"

    Article Title: GETPrime: a gene- or transcript-specific primer database for quantitative real-time PCR

    Journal: Database: The Journal of Biological Databases and Curation

    doi: 10.1093/database/bar040

    JBrowse-based graphical view of GETPrime primer pairs targeting the Rbbp9 mouse gene. The blue boxes on the left are the available tracks that can be dragged in the JBrowse genome view . In this example, the transcripts, the gene-specific primers (covering the majority of splice variants if possible) and the transcript-specific primers (covering a single splice variant, when possible) have been dragged into the browser. The upper part of the figure shows tools to zoom, to move to up- or downstream of the genome location, and to enter another chromosome, another position on the chromosome or also an Ensembl ID. Each primer is annotated by its Ensembl ID, its iteration in GETPrime (e.g. −1), its ranking (e.g. _3) and its primer type (forward and reverse primers are abbreviated Fwd and Rv, respectively). The blue box for each primer represents the respective alignment to the transcripts and sometimes a thin line between two blue boxes is used to bridge an intron region for primers spanning two exons. The primer pairs in the gene-specific track cover both transcripts. The primer pairs from the first iteration (‘−1’) and the second iteration (‘−2’) in the transcript-specific track are specific to the largest transcript Rbbp9-001 and the shortest transcript Rbbp9-002 , respectively.
    Figure Legend Snippet: JBrowse-based graphical view of GETPrime primer pairs targeting the Rbbp9 mouse gene. The blue boxes on the left are the available tracks that can be dragged in the JBrowse genome view . In this example, the transcripts, the gene-specific primers (covering the majority of splice variants if possible) and the transcript-specific primers (covering a single splice variant, when possible) have been dragged into the browser. The upper part of the figure shows tools to zoom, to move to up- or downstream of the genome location, and to enter another chromosome, another position on the chromosome or also an Ensembl ID. Each primer is annotated by its Ensembl ID, its iteration in GETPrime (e.g. −1), its ranking (e.g. _3) and its primer type (forward and reverse primers are abbreviated Fwd and Rv, respectively). The blue box for each primer represents the respective alignment to the transcripts and sometimes a thin line between two blue boxes is used to bridge an intron region for primers spanning two exons. The primer pairs in the gene-specific track cover both transcripts. The primer pairs from the first iteration (‘−1’) and the second iteration (‘−2’) in the transcript-specific track are specific to the largest transcript Rbbp9-001 and the shortest transcript Rbbp9-002 , respectively.

    Techniques Used: Variant Assay

    Graphical view and qPCR results to validate Ubtf -targeting primers covering either all or a subset of Ubtf transcripts. ( A ) The ‘Ubtf’ primer pair in blue covers all seven transcripts (gene-specific primers) and the red ‘Ubtf_a’ and ‘Ubtf_b’ primer pairs cover five and two transcripts, respectively (transcript-specific primers). In this example, GETPrime could not find primers differentiating each transcript. ( B ) The relative gene expression levels before differentiation (D0) and four days after (D4) were normalized to Hprt1 and Tubb2c expression levels. ‘Ubtf’ represents the primer pair covering all seven transcripts, whereas, ‘Ubtf_a’ and ‘Ubtf_b’ are primer pairs specific to a subset of five and two transcripts, respectively. ‘Ubtf_a+Ubtf_b’ represents the sum of relative gene expression of ‘Ubtf_a’ and ‘Ubtf_b’. The data indicate that GETPrime can effectively differentiate distinct transcripts, as the sum of the individual transcript amounts matched the overall gene expression amount.
    Figure Legend Snippet: Graphical view and qPCR results to validate Ubtf -targeting primers covering either all or a subset of Ubtf transcripts. ( A ) The ‘Ubtf’ primer pair in blue covers all seven transcripts (gene-specific primers) and the red ‘Ubtf_a’ and ‘Ubtf_b’ primer pairs cover five and two transcripts, respectively (transcript-specific primers). In this example, GETPrime could not find primers differentiating each transcript. ( B ) The relative gene expression levels before differentiation (D0) and four days after (D4) were normalized to Hprt1 and Tubb2c expression levels. ‘Ubtf’ represents the primer pair covering all seven transcripts, whereas, ‘Ubtf_a’ and ‘Ubtf_b’ are primer pairs specific to a subset of five and two transcripts, respectively. ‘Ubtf_a+Ubtf_b’ represents the sum of relative gene expression of ‘Ubtf_a’ and ‘Ubtf_b’. The data indicate that GETPrime can effectively differentiate distinct transcripts, as the sum of the individual transcript amounts matched the overall gene expression amount.

    Techniques Used: Gene Expression, Expressing



    Similar Products

    qpcr primer design software getprime  (PrimerDesign Inc)
    90
    PrimerDesign Inc qpcr primer design software getprime
    JBrowse-based graphical view of <t>GETPrime</t> primer pairs targeting the Rbbp9 mouse gene. The blue boxes on the left are the available tracks that can be dragged in the JBrowse genome view . In this example, the transcripts, the gene-specific primers (covering the majority of splice variants if possible) and the transcript-specific primers (covering a single splice variant, when possible) have been dragged into the browser. The upper part of the figure shows tools to zoom, to move to up- or downstream of the genome location, and to enter another chromosome, another position on the chromosome or also an Ensembl ID. Each primer is annotated by its Ensembl ID, its iteration in GETPrime (e.g. −1), its ranking (e.g. _3) and its primer type (forward and reverse primers are abbreviated Fwd and Rv, respectively). The blue box for each primer represents the respective alignment to the transcripts and sometimes a thin line between two blue boxes is used to bridge an intron region for primers spanning two exons. The primer pairs in the gene-specific track cover both transcripts. The primer pairs from the first iteration (‘−1’) and the second iteration (‘−2’) in the transcript-specific track are specific to the largest transcript Rbbp9-001 and the shortest transcript Rbbp9-002 , respectively.
    Qpcr Primer Design Software Getprime, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qpcr+primer+design+software+getprime/pmc03173022-43-13-10?v=PrimerDesign+Inc
    Average 90 stars, based on 1 article reviews
    qpcr primer design software getprime - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    JBrowse-based graphical view of GETPrime primer pairs targeting the Rbbp9 mouse gene. The blue boxes on the left are the available tracks that can be dragged in the JBrowse genome view . In this example, the transcripts, the gene-specific primers (covering the majority of splice variants if possible) and the transcript-specific primers (covering a single splice variant, when possible) have been dragged into the browser. The upper part of the figure shows tools to zoom, to move to up- or downstream of the genome location, and to enter another chromosome, another position on the chromosome or also an Ensembl ID. Each primer is annotated by its Ensembl ID, its iteration in GETPrime (e.g. −1), its ranking (e.g. _3) and its primer type (forward and reverse primers are abbreviated Fwd and Rv, respectively). The blue box for each primer represents the respective alignment to the transcripts and sometimes a thin line between two blue boxes is used to bridge an intron region for primers spanning two exons. The primer pairs in the gene-specific track cover both transcripts. The primer pairs from the first iteration (‘−1’) and the second iteration (‘−2’) in the transcript-specific track are specific to the largest transcript Rbbp9-001 and the shortest transcript Rbbp9-002 , respectively.

    Journal: Database: The Journal of Biological Databases and Curation

    Article Title: GETPrime: a gene- or transcript-specific primer database for quantitative real-time PCR

    doi: 10.1093/database/bar040

    Figure Lengend Snippet: JBrowse-based graphical view of GETPrime primer pairs targeting the Rbbp9 mouse gene. The blue boxes on the left are the available tracks that can be dragged in the JBrowse genome view . In this example, the transcripts, the gene-specific primers (covering the majority of splice variants if possible) and the transcript-specific primers (covering a single splice variant, when possible) have been dragged into the browser. The upper part of the figure shows tools to zoom, to move to up- or downstream of the genome location, and to enter another chromosome, another position on the chromosome or also an Ensembl ID. Each primer is annotated by its Ensembl ID, its iteration in GETPrime (e.g. −1), its ranking (e.g. _3) and its primer type (forward and reverse primers are abbreviated Fwd and Rv, respectively). The blue box for each primer represents the respective alignment to the transcripts and sometimes a thin line between two blue boxes is used to bridge an intron region for primers spanning two exons. The primer pairs in the gene-specific track cover both transcripts. The primer pairs from the first iteration (‘−1’) and the second iteration (‘−2’) in the transcript-specific track are specific to the largest transcript Rbbp9-001 and the shortest transcript Rbbp9-002 , respectively.

    Article Snippet: To fill this current void, we developed our own qPCR primer design software, GETPrime.

    Techniques: Variant Assay

    Graphical view and qPCR results to validate Ubtf -targeting primers covering either all or a subset of Ubtf transcripts. ( A ) The ‘Ubtf’ primer pair in blue covers all seven transcripts (gene-specific primers) and the red ‘Ubtf_a’ and ‘Ubtf_b’ primer pairs cover five and two transcripts, respectively (transcript-specific primers). In this example, GETPrime could not find primers differentiating each transcript. ( B ) The relative gene expression levels before differentiation (D0) and four days after (D4) were normalized to Hprt1 and Tubb2c expression levels. ‘Ubtf’ represents the primer pair covering all seven transcripts, whereas, ‘Ubtf_a’ and ‘Ubtf_b’ are primer pairs specific to a subset of five and two transcripts, respectively. ‘Ubtf_a+Ubtf_b’ represents the sum of relative gene expression of ‘Ubtf_a’ and ‘Ubtf_b’. The data indicate that GETPrime can effectively differentiate distinct transcripts, as the sum of the individual transcript amounts matched the overall gene expression amount.

    Journal: Database: The Journal of Biological Databases and Curation

    Article Title: GETPrime: a gene- or transcript-specific primer database for quantitative real-time PCR

    doi: 10.1093/database/bar040

    Figure Lengend Snippet: Graphical view and qPCR results to validate Ubtf -targeting primers covering either all or a subset of Ubtf transcripts. ( A ) The ‘Ubtf’ primer pair in blue covers all seven transcripts (gene-specific primers) and the red ‘Ubtf_a’ and ‘Ubtf_b’ primer pairs cover five and two transcripts, respectively (transcript-specific primers). In this example, GETPrime could not find primers differentiating each transcript. ( B ) The relative gene expression levels before differentiation (D0) and four days after (D4) were normalized to Hprt1 and Tubb2c expression levels. ‘Ubtf’ represents the primer pair covering all seven transcripts, whereas, ‘Ubtf_a’ and ‘Ubtf_b’ are primer pairs specific to a subset of five and two transcripts, respectively. ‘Ubtf_a+Ubtf_b’ represents the sum of relative gene expression of ‘Ubtf_a’ and ‘Ubtf_b’. The data indicate that GETPrime can effectively differentiate distinct transcripts, as the sum of the individual transcript amounts matched the overall gene expression amount.

    Article Snippet: To fill this current void, we developed our own qPCR primer design software, GETPrime.

    Techniques: Gene Expression, Expressing